Practical Essay Example

Practical Essay Practical Name: Institution: Course: We will write a custom essay sample on Practical specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Practical specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Practical specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Date: Practical Introduction The aim of embryo culture is to obtain a plant after isolating and growing an embryo in vitro. It involves the cultivation of embryos from seeds and ovules in aseptic conditions. The culture medium depends on the type of plants, and the age of the embryo. A person can use mature or immature embryos. People practice embryo culture for different reasons. Some do it because they want to know more about the plant. Growing the embryos outside the ovules gives a person the chance to learn the nutrient composition of the plant at every stage of development, identify the requirements of the plant and identify the different stages of growth. Embryo culture provides a way of getting hybrid seedlings and overcoming seed dormancy. This enables the production of crops with superior qualities. The practice of embryo culture is important because it used to recover distant hybrids and haploid plants. This practice is important because it shortens the breeding cycle of the plants (Bhojwani Razdan, 1 996). Materials and Methods We used snow peas pods and five plates of pea culture medium. We adjusted the culture medium to a pH of 5.7. Embryos grow well in a medium with a pH of 5 to 7 (Razdan, 2003). It contained 110g/L of vitamins, 11% sucrose, 50ml/L of coconut water containing agar, and casein hydrolysale. One needs to select the right culture medium that will support orderly and progressive development of the embryo. One should ensure that the medium is able to nourish the embryo. Sucrose is a source of energy and it supplements the vitamins. Using high quantity of vitamins can inhibit the growth. Therefore, one should use vitamins minimally. Other materials included scalpels, plates, grid paper, and aluminum foil. We rinsed the pods with water, and then sterilized them in 70% ethanol for two minutes. We then washed the pods again with distilled water and treated them with 2% hypochlorite solution. After the treatment, we washed the seeds again using distilled water. We opened the cotyledons, and removed the embryo hearts from the snow peas pods while exercising caution not to damage the embryos. We used a scalpel tip to cut the embryos horizontally into two parts. We measured the embryos using a grid paper. It is important and mandatory to measure the embryos before putting them in culture, so that one can determine the level of growth after the appointed time. We then photographed the embryos using the digital camera. We then put the two embryos in different culture media. We sealed the plates with the aluminum foil and incubated them at 22o for three weeks. After this time, we re-measured the embryos to check their growth. Results Embryo culture results- Initial Size (mm2)Final size (mm2)Difference (mm2) 2 25 23 2 25 23 3 35 32 1 10 9 4 20 16 9 30 21 9 25 16 10 30 20 8 36 28 12 150 138 4 60 56 Discussion There was only one healthy cotyledon. The other cotyledons were contaminated in different ways or damaged by heat. The contamination of the embryos may have happened during the cutting process. Although we sterilized and treated the embryos, we did not take the same effort in ensuring that the materials we were using were not contaminated. This affects the results of the culture. We did not get the results we had anticipated. The embryos did not grow as well as we had expected them to do. Some of the embryos were damaged by heat. We had sealed them with aluminum foil and put them under 22o. It seems that we exaggerated the level of heat required, or we used the wrong materials to seal the embryos. Contamination affects the growth of the embryos because it introduces bacteria. The bacteria breed on the embryos, and destroy the cells of the embryos. This hinders growth. We were not careful when extracting the embryos from the pods, and we saw this after seeing some of the damaged cotyl edons. This destroyed the embryonic tissues and prohibited the embryos development. We had used embryos of different sizes, ranging from 1 mm2 to 12 mm2. At the end of the experiment, the sizes of the final cotyledons did not correspond to the sizes of the embryos. However, we experienced the highest growth rate of 150 mm2 from the largest embryo, and the lowest growth rate of 10 mm2 from the smallest embryo. It seems that the contamination and the heat affected the growth of the embryos. Growth of the embryos occurs when there is cell division in the embryos. The development of the embryos occurs when cells specialize. This explains why there was growth in the cells, although they were contaminated, and why the largest embryos showed the highest growth rates. The size of the embryos determines the growth rate of the embryos. Large embryos have many cells, while the smaller embryos have fewer cells. Therefore, the larger embryos have a higher chance of developing (Kohl, 2007). References: Bhojwani, S. S., Razdan, K. M. (1996). Plant tissue culture: Theory and practice. Boston, MA: Elsevier Kohl, B. (2007). Embryo culture: Making babies in the twenty-first century. New York, NY: Farrar, Straus and Giroux Razdan, K. M. (2003). Introduction to plant tissue culture. Enfield, NH: Science Publishers, Inc.

How to Care for Horses

How to Care for Horses Free Online Research Papers Emir Abd-el-Kader once said â€Å"By reason of his elegance, he resembles an image painted in a palace, though he is as majestic as the palace itself.† Horses are an important part of the world today. Their elegance, style beauty and grace make them one of the most favorable animals in the world and caring for them can be for pleasure or career. There are many factors in caring for the horses such as housing, nutrition, medical attention, exercise and blacksmith visits. To own a horse you must have the proper housing. Nowadays horses are mainly kept in a stable for most of their lives. This could be for convenience or so they are kept neat and tidy for shows. The horse must be provided ample room so they do not get cast (horse lies down too close to a wall and cannot get up). The standard measurement for a stall would be 12 feet by 12 feet. (Val Equine net 2006). Although it is not a necessity many horses do better when they are allowed pasture to graze upon. If there are multiple horses outside together it is essential to have 2 acres of land per horse. Horses also need proper nutrition. They should be provided water free choice(unlimited supply) and they should drink about 12 to 20 gallons per day. If you plan on keeping your horse in a stall you will need to refill the water bucket multiple times throughout the day, unless you have an automated water system or as needed. There are many different types of feed that are available but personally I would choose Nutrena Safe Choice Horse Feed because of it has high levels of protein and carbohydrates. You will also need hay. Orchard grass is usually the cheapest but you might want to use an alfalfa depending on the age and condition of the horse. Horses that are outside and being maintained as a show animal will need their pasture regulated (how much grass they consume) so they do not gain too much weight. Horses must have their vaccinations just as people would. There are many different types of vaccinations such as botulism, encephalomyelitis, influenza, Potomac horse fever; rabies and strangles just to name a few (Prinz 2005). Your horse will receive its first shots a few days after it is born and then will continue to need the vaccinations throughout his/her life. They also need to be dewormed every three to six weeks. You should always plan your whole year’s worming routine and mark each tube and the date to be given. This will help you to not miss any critical dates that might change the cycle.(Hayes 1999) Exercise is a vital part of the horse’s daily routine. There are lots of reasons for exercising horses kept in stalls besides keeping them mentally and physically healthy. Researchers from the University of Flordia have found improved leg bone and joint development in foals that were stalled but given regular exercise. Foals that were stalled without exercise rebounded in bone development when they were turned out to pasture. Weanlings stalled without exercise had less bone mass than those left on pasture. In older horses you must exercise to prevent illnesses such as colic. Ways of exercise include riding, lunging walking, and turnout(putting a horse outside for self exercise). Another thing that horses need is hoof care. A horses hooves should be routinely cared for every four to six weeks (Johnson 2003). There are many factors that should take into account while deciding when the time is right such as health of hooves, potential anticipated use, defects in gait and/or conformation and the injuries of the horse. If your horse is conformationally correct then the hooves should just be trimmed. However if there is a conformation fault or the horse is being used a lot(showing every weekend, etc.) you will need corrective shoeing which either fixes the conformation faults or lets the horse experience less shock onto the hooves. Although costly owning a horse can provide joy into your life. Everyday caring for the horse will give you satisfaction. It may seem like a lot of work in the beginning but eventually you will get into a routine. Yet you can never stop learning. Your horse should live in a clean, healthy environment cared for as if he/she were a king. Research Papers on How to Care for HorsesWhere Wild and West MeetResearch Process Part OneGenetic EngineeringThe Spring and AutumnThe Masque of the Red Death Room meaningsMarketing of Lifeboy Soap A Unilever ProductThe Effects of Illegal ImmigrationPersonal Experience with Teen PregnancyThe Hockey GameBionic Assembly System: A New Concept of Self

Review emerging standards of culturally competent care Research Paper

Review emerging standards of culturally competent care - Research Paper Example This is possible only if health professionals are sensitive enough to demonstrate respect toward the different values, beliefs, and practices of one’s counterparts (Cherry & Jacob, 2005, p.246). The writers also insist on the need of taking responsibility ‘to inquire, learn about, and integrate beliefs and values of others in professional encounters’ so that it would change one’s negative prejudicial behavior. This paper will evaluate the significance of developing cultural competence in nursing care especially in critical care settings like Intensive care units (ICU) where proficient care is highly crucial. What is Culturally Competent Care? Health care is a very sensitive area of service where people often confront with long standing disparities on the basis of culture, tradition, nationality, ethnicity, language, and socio-economic status. ‘Culturally Competent Care’ is the modern perception that has been developed to address health and huma n service issues in a multicultural social environment. In other words, the modern world requires collective responses of doctors, nurses, and other care givers to patient’s varying attitudes, values, verbal indications, and many more; which is possible only by initiating cultural sensitivity as a new dimension of clinical specialization like age and gender. In order to be culturally competent, nurse needs to obtain cultural information with intent to apply that knowledge into practice while they encounter various sorts of ethnic and cultural differences. The major objective of this knowledge acquisition is to avoid stereotyping patients on the basis of their race, culture, or any external appearance. The logic behind this approach is that high-quality outcome can be derived from culturally competent care. When doctors and nurses familiarize themselves with the cultural perceptions and practices of patient, normally it would make a positive impact on treatment results and pat ient satisfaction. Why cultural competence in nursing? As mentioned in the introduction, cultural diversity has become the major facet of almost every developed nation. Therefore, in order to meet the specific needs of people with different cultural background, the nursing care given should be culturally competent. Health care industry reform has been a hot button issue for several decades in the United Sates. Although several attempts have been made to equalize care delivery across the nation, as critics comment, all were defeated by unethical medical-political coalition. Culture influences people’s perception of illness and their causes, the way they seek health care, their approaches to medical treatments, and how they respond to health care providers. Therefore, health providers especially nurses need to obtain skills to act according to the health behaviors of the clients. Infant death and pregnancy loss is an ongoing problem in the United States of America. Studies show the noticeable racial and community based disparities in infant death rates. This situation points to the need of equal delivery of health care services to everyone regardless the race, culture, or community. According to the AAN Expert Panel Report (1992) culturally competent nursing care should follow mainly four principles; 1) care is designed for the specific client 2) based on the uniqueness of the

Early Greek Legacy - Choice of topics listed Essay

Early Greek Legacy - Choice of topics listed - Essay Example did not have any belief in material image of God- no creature can represent the creator. Whereas in Eastern religions like Hinduism, the material images of God is worshiped. They believed in the personality of human of being in God like he could be angry, even jealous, but his primary attributes were righteousness, justice mercy, truth and faithfulness. He is represented as king, Judge and shepherd. Whereas in Hinduism various deities are worshiped at shrines; the divine trinities, representing the cyclical nature of the universe, are Brahma the creator, Vishnu the preserver, and Shiva the destroyer. The first books of Hebrew law contain legal writings of Exodus, Leviticus, and Numbers. The fifth book of the Bible was called Deuteronomy [Second Law] primarily a report of the last words and deeds of Moses. It contains numerous laws, often in the context of interpretation and preaching whereas Hindu law preaches the birth, death and rebirth of living things, and its precepts cover many more activities than does any secular legal system. The Hebrew people gave Torah special reverence than the portion of the Hebrew Bible because the written Torah which consists of the five books of Moses, Genesis, Exodus, Leviticus, Numbers, and Deuteronomy .The scrolls are considered mostly holy. Whereas the Hebrew Bible which is also called as the Jewish Bible even though it consists of Hebrew Scriptures, 39 books originally written in Hebrew, except for a few sections in Aramaic, the script of Hebrew Bible was not given importance while compared to Torah which valued the tradition without any modification. In the case of the covenant between God and the Hebrew people, God’s obligation is conceived as the defense or vindication of the people .His righteousness is shown by his saving arts [see, for instance ,psalms 98:2;Isaiah 51:5] Hebrew’s obligation on the other hand ,is to obey the will of God as disclosed in the Torah, the law. Yes, each party honored the

(Social) Health service needs of young people (aged 1830) attending Essay

(Social) Health service needs of young people (aged 1830) attending social care services (at ACCESS) in the Cottonera area ( in Malta) - Essay Example Literature in the realm of research performed on the advances in social healthcare and their use would be quite numerous and diverse in their content and methodology. In the different separate studies located, only few would have spanned a time beyond a decade. The majority of research pieces, which focus on the inclusion of theoretical and technological advances in the sales and marking process, would actually focus on the overall social segments through fundamental health and caring levels. The fact that there is not very many-advanced research items specifically directed toward the social strategy and healthcare in the context of Social Health service needs of young people (aged 18-30) attending social care services (at ACCESS) in the Cottonera area in Malta would be the focus of this proposal. Malta's average population density is almost 1282 person per square km, which is very high. It also has a huge number of unemployed people and school dropouts surviving only on social benefits. They have the lowest educational achievement rates and ambitions in the region. There are also high levels of absence and truancy among the students. This results in most of the people living in mediocre and insufficient housing conditions. The Cottonera locality is among the utmost population density regions having a gradually aging population as most couples leave the locality for a better life elsewhere. Their total fertility rate is low at 1.37 and there has been a downward inclination in the total number of childbirths in the past 10 years. The proportion of children under 15 is rapidly declining while the proportion of people above 65 is increasing. Their unemployment rate, which is 53.9%, is also very high. Also the rate of participation of women in the workforce is lower than that of men. In order to eliminate these challenges, the Maltese government needs to prevent poverty and harbor the potential of it young people and children. ACCESS attempts to address these intricate social and cultural issues in a holistic, multidisciplinary, community based and client-centered method. Among the responsibilities of ACCESS is to create equal opportunities for people with less privileged backgrounds so as to reduce poverty and social barring between generations. It also promotes public engagement between the residents, which helps to tackle the downbeat self-image held by the different community members who take themselves to be failures. It intervenes at different steps in the life of the people through services and projects in order to break the sequence resulting in reproduction by successive generations of parents' position having limited opportunities and social elimination. The ACCESS staff

Pelvic Inflammatory Disease Diagnosis And Management Nursing Essay

Pelvic Inflammatory Disease Diagnosis And Management Nursing Essay Pelvic inflammatory disease is an infection which causes wide variety of infection from upper to lower genital tract.(1) It ascends from cervix or vagina to peritoneal cavity include endometritis, salpingitis, parametritis, oophoritis, tuboovarian abscess and pelvic peritonitis. (2,3) PID is a major problem in public health consequences because it is related to fallopian tube inflammation which can lead to infertility as a final complication.(1) PID is polymicrobial disease, so some sexually transmitted microorganisms are associated with PID. These are Chlamydia trachomatis, Mycoplasma genitalium, Neisseria gonorrhoea, and bacterial vaginosis, predominantly anaerobes. (4, 7) PID can be prevent by regular screening for Chlamydia infection and appropriate treatment of it. (4) There is no single diagnosis or finding that can do specific diagnosis of PID. The diagnosis is based on the result of pelvic organ tenderness. Mild-to-moderate PID patients are treat as out patient which include tolerated antibiotic regimens against common microorganism in PID Clinically severe PID treatment done as hospitalization of the patient. (11) Sexually active women especially at the reproductive age and under the age of 25, are at the highest risk for acquiring this disease through sexually transmitted bacteria. The intrauterine devices (IUDs) are also increased risk in women who have this device in their uterus for contraception.(6) Pic: The female anatomy (6) Differential diagnosis: In case of PID the clinician should concern about differential diagnosis before confirm the diagnosis. The differential diagnosis PID of can be as follows (3): Ectopic pregnancy Endometritis Salpingitis(8) Cervicitis (8) Ovarian cyst torsion, rapture or malignancy UTI Appendicitis Clinical Diagnosis of PID: Clinical diagnosis of PID is based on the combination of patients clinical history, physical examination and some laboratory studies.(2,5) The following findings are important for diagnosis of PID: Physical or general finding: Low grade temperature, lower abdominal pain, abnormal intermenstrual bleeding or metrorrhagia, abnormal cervical discharge, postcoital pain and bleeding, urinary frequency, low back pain, nausea and vomiting.(5,8) Bimanual pelvic examination: Cervical and uterine motion tenderness or adnexal tenderness should present for confirming the diagnosis of PID.(10). Laboratory finding: Leucocytosis more than 10 x 109 WBC/L , elevated C-reactive protein, elevated ESR, Gram negative intracellular diplococcic on gram stain, and positive Chlamydia test.(5) Some definitive diagnosis: Endometrial biopsy for endometritis, transvaginal sonography or ultrasonography for pelvic or tubo-ovarian complex and the laparoscopic abnormalities associated with PID.(5) Management The PID management include short term and long term treatment. Short term treatment help to reduce or eliminate the sign symptom of patients. On the other hand long term treatment help to decrease the further complications. Outpatient therapy: As aforementioned that mild-to-moderate PID patients are given treatment as out patient therapy (5) Recommended Regimen: ceftriaxone 250 mg im as one dose + doxycycline 100 mg orally 12 hourly for 14 days or Azithromycin 500 mg orally followed by 250 mg orally daily for a total of 7 days + metronidazole 400 mg orally 12 hourly for 14 days Inpatient therapy: Clinically severe PID treatment done as hospitalization of the patient. (11) Recommended Regimen(5): Clindamycin 900 mg intravenously every 8 hours for 14 days PLUS Ceftriaxone 1g intravenously every 12 hours for 14 days Alternative Regimens(12) Cefoxitin 2 g intravenously every 6 hours for 14 days OR Cefotetan 2 g intravenously every 12 hours for 14 days PLUS Doxycycline 100 mg orally or intravenously every 12 for 14 days hours OR Ampicillin/sulbactam 3 g intravenously every 6 hours for 14 days PLUS Doxycycline 100 mg orally or intravenously every 12 hours for 14 days Indications for hospitalisation If the patients are required intravenous therapy for serious clinical condition, then patient should be hospitalised. The following patients should be hospitalised, clinically severe patients, pregnant woman with PID, surgical emergency such as appendicitis, ectopic pregnancy, failure of out patient therapy and immunodeficiency patient. (5) Removal of IUCD The intrauterine devices (IUDs) increased the risk of PID. So IUCD should remove if there is any clinical evidence of PID.(6) Complication of PID: Delay in diagnosis and treatment, or inadequate treatment increase the rate of complications.(13) The complications are (13): Chronic lower abdominal pain Ectopic pregnancy Increased risk of PID in future Tubo-ovarian abscess. Infertility The points should known to patients (5): It can be acquired other than sexually transmitted and the partners also should be tested and treated for sexually transmitted infections. The nature of infection and complication should be known to patients and they should know the importance of follow up. Contact tracing Contact tracing is finding and notifying the person with the infection so they can have counselling, testing and treatment and it is important for prevention the long term health problem.(9) Follow-up Close follow up is necessary for prevention of complications.(5) Prevention: Prevention of STD is necessary to prevent PID. So early detection of any lower genital infections is necessary to prevent PID. (14) Cervical Chlamydial infection identification and treating can make smaller the incidence number of PID. (4) Finally, sex partners of women with PID should be examined and treated for gonococcal and Chlamydial infection for prevention the spread of STDs in the community.(14)

The Function of Blogs :: Internet

The Function of Blogs There are so many things one could do with the relatively new medium of blogs. They can be almost anything a person wants: a journal, a compilement of links, a scrapbook of information, or even a discussion board. The only definition I've seen that can encompass it is that they are postings in reverse chronological order. Blogs are the newest Internet craze, but do they serve a purpose? Early on, experienced web users, who knew web-programming language, kept blogs to keep track of their mind’s wanderings. They were like a "Captains log on the quest of discovery" (Brown). For the average Internet surfer, they weeded out sites worth viewing from the rest. Now, sites like blogger.com have taken the work with web languages out of blogging, opening it up to the general public. New age blogs are seen as "vain self publishing" by experienced bloggers (Fitcher). I can see their point, as many inexperienced bloggers seem to ramble on about themselves as if they are to be praised. They only seem to want attention. This has made me wonder, what is the point of personal blogs? All blogs must have some entertainment value; otherwise no one would keep or read them. Entertainment isn’t a very commendable purpose, but it has more value than watching television. It is better to be learning about news events and stating your opinion on it than just hearing it. At least then you’re developing your ideas, and the reading and writing will help develop communication skills. Bloggers learn to put more thought into what they say, or just the act of writing daily can help thoughts flow into words. A blogger’s thoughts may stem from a news article they recently read. In this case they usually link you to the article and state their opinions, or point out different aspects of it. Their comments are kind of a one-way discussion leading you down their train of thoughts. You can usually reply to what the author says, but the time delay in responses may result in the author’s lack of interest to questions posed. Walt Crawford threw an interesting concept my way in his article, Announcing Cubed: Media about Media about Media. Blogs are just another form of media coverage. Our media affects how we think to a great extent. It is always rating other forms of media, and many blogs are no exception (Crawford).